The points leader has been docked 25 points in the drivers' standings, while his team has been docked the same amount in the owners' standings, after officials found an improperly attached weight on the #18 car during the opening day inspection.
Jason Ratcliff, the team's crew chief, has been fined $5,000 and placed on probation until the end of the year for the rules infringement.
Despite the points loss, Busch can still clinch his maiden NASCAR title next weekend at Phoenix, but he now needs to finish seventh or better on Saturday in order to become champion.
Primera Liga round-upKyle Busch and team penalised